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Promega pglosensor-22f camp plasmid e2301
Pglosensor 22f Camp Plasmid E2301, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pglosensor-22f+camp+plasmid+e2301/pglosensor+22f+camp+plasmid/pmc11474063__pnas__2407130121__sapp-82-27-30
Average 90 stars, based on 1 article reviews
pglosensor-22f camp plasmid e2301 - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Plasmid Preparation:

Article Title: Compartment-Specific Activation of the Proton-Sensor GPR65 is Uncoupled from Receptor Trafficking
Article Snippet: The following amounts of DNA were used per well: 60 ng of pGloSensor-22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control, pCDNA3.1 + ).

Article Title: SPMs exert anti-inflammatory and pro-resolving effects through positive allosteric modulation of the prostaglandin EP4 receptor
Article Snippet: To generate a lentiviral transfer plasmid (pLV) carrying the cDNA encoding the GloSensor-22F cAMP reporter, the coding sequence of the GloSensor-22F cAMP reporter was subcloned from the pGloSensor-22F cAMP plasmid (Promega, E2301) into the backbone of basic pLV-puro lentiviral expression vector (Vector builder).

Article Title: Diversely evolved xibalbin variants from remipede venom inhibit potassium channels and activate PKA-II and Erk1/2 signaling.
Article Snippet: B) HEK293T cells were transfected with pGloSensor‐22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany). cAMP transfected HEK293T cells were incubated in DMEM without phenol red supplemented with pGlo sensor cAMP reagent (E1290, Promega, Walldorf, Germany).

Article Title: Diversely evolved xibalbin variants from remipede venom inhibit potassium channels and activate PKA-II and Erk1/2 signaling
Article Snippet: B) HEK293T cells were transfected with pGloSensor-22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany). cAMP transfected HEK293T cells were incubated in DMEM without phenol red supplemented with pGlo sensor cAMP reagent (E1290, Promega, Walldorf, Germany).

Article Title: The Pharmacological Potential of Novel Melittin Variants from the Honeybee and Solitary Bees against Inflammation and Cancer
Article Snippet: HEK293T cells were transfected with pGloSensor-22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany).

Article Title: Location-biased activation of the proton-sensor GPR65 is uncoupled from receptor trafficking.
Article Snippet: The following amounts of DNA were used per well: 60 ng of pGloSensor- 22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control, pCDNA3.1+).

Sequencing:

Article Title: Compartment-Specific Activation of the Proton-Sensor GPR65 is Uncoupled from Receptor Trafficking
Article Snippet: The following amounts of DNA were used per well: 60 ng of pGloSensor-22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control, pCDNA3.1 + ).

Article Title: SPMs exert anti-inflammatory and pro-resolving effects through positive allosteric modulation of the prostaglandin EP4 receptor
Article Snippet: To generate a lentiviral transfer plasmid (pLV) carrying the cDNA encoding the GloSensor-22F cAMP reporter, the coding sequence of the GloSensor-22F cAMP reporter was subcloned from the pGloSensor-22F cAMP plasmid (Promega, E2301) into the backbone of basic pLV-puro lentiviral expression vector (Vector builder).

Article Title: Diversely evolved xibalbin variants from remipede venom inhibit potassium channels and activate PKA-II and Erk1/2 signaling.
Article Snippet: B) HEK293T cells were transfected with pGloSensor‐22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany). cAMP transfected HEK293T cells were incubated in DMEM without phenol red supplemented with pGlo sensor cAMP reagent (E1290, Promega, Walldorf, Germany).

Article Title: Diversely evolved xibalbin variants from remipede venom inhibit potassium channels and activate PKA-II and Erk1/2 signaling
Article Snippet: B) HEK293T cells were transfected with pGloSensor-22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany). cAMP transfected HEK293T cells were incubated in DMEM without phenol red supplemented with pGlo sensor cAMP reagent (E1290, Promega, Walldorf, Germany).

Article Title: The Pharmacological Potential of Novel Melittin Variants from the Honeybee and Solitary Bees against Inflammation and Cancer
Article Snippet: HEK293T cells were transfected with pGloSensor-22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany).

Article Title: Location-biased activation of the proton-sensor GPR65 is uncoupled from receptor trafficking.
Article Snippet: The following amounts of DNA were used per well: 60 ng of pGloSensor- 22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control, pCDNA3.1+).

Expressing:

Article Title: Compartment-Specific Activation of the Proton-Sensor GPR65 is Uncoupled from Receptor Trafficking
Article Snippet: The following amounts of DNA were used per well: 60 ng of pGloSensor-22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control, pCDNA3.1 + ).

Article Title: SPMs exert anti-inflammatory and pro-resolving effects through positive allosteric modulation of the prostaglandin EP4 receptor
Article Snippet: To generate a lentiviral transfer plasmid (pLV) carrying the cDNA encoding the GloSensor-22F cAMP reporter, the coding sequence of the GloSensor-22F cAMP reporter was subcloned from the pGloSensor-22F cAMP plasmid (Promega, E2301) into the backbone of basic pLV-puro lentiviral expression vector (Vector builder).

Article Title: Diversely evolved xibalbin variants from remipede venom inhibit potassium channels and activate PKA-II and Erk1/2 signaling.
Article Snippet: B) HEK293T cells were transfected with pGloSensor‐22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany). cAMP transfected HEK293T cells were incubated in DMEM without phenol red supplemented with pGlo sensor cAMP reagent (E1290, Promega, Walldorf, Germany).

Article Title: Diversely evolved xibalbin variants from remipede venom inhibit potassium channels and activate PKA-II and Erk1/2 signaling
Article Snippet: B) HEK293T cells were transfected with pGloSensor-22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany). cAMP transfected HEK293T cells were incubated in DMEM without phenol red supplemented with pGlo sensor cAMP reagent (E1290, Promega, Walldorf, Germany).

Article Title: The Pharmacological Potential of Novel Melittin Variants from the Honeybee and Solitary Bees against Inflammation and Cancer
Article Snippet: HEK293T cells were transfected with pGloSensor-22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany).

Article Title: Location-biased activation of the proton-sensor GPR65 is uncoupled from receptor trafficking.
Article Snippet: The following amounts of DNA were used per well: 60 ng of pGloSensor- 22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control, pCDNA3.1+).

Transfection:

Article Title: Compartment-Specific Activation of the Proton-Sensor GPR65 is Uncoupled from Receptor Trafficking
Article Snippet: The following amounts of DNA were used per well: 60 ng of pGloSensor-22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control, pCDNA3.1 + ).

Article Title: SPMs exert anti-inflammatory and pro-resolving effects through positive allosteric modulation of the prostaglandin EP4 receptor
Article Snippet: To generate a lentiviral transfer plasmid (pLV) carrying the cDNA encoding the GloSensor-22F cAMP reporter, the coding sequence of the GloSensor-22F cAMP reporter was subcloned from the pGloSensor-22F cAMP plasmid (Promega, E2301) into the backbone of basic pLV-puro lentiviral expression vector (Vector builder).

Article Title: Diversely evolved xibalbin variants from remipede venom inhibit potassium channels and activate PKA-II and Erk1/2 signaling.
Article Snippet: B) HEK293T cells were transfected with pGloSensor‐22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany). cAMP transfected HEK293T cells were incubated in DMEM without phenol red supplemented with pGlo sensor cAMP reagent (E1290, Promega, Walldorf, Germany).

Article Title: Diversely evolved xibalbin variants from remipede venom inhibit potassium channels and activate PKA-II and Erk1/2 signaling
Article Snippet: B) HEK293T cells were transfected with pGloSensor-22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany). cAMP transfected HEK293T cells were incubated in DMEM without phenol red supplemented with pGlo sensor cAMP reagent (E1290, Promega, Walldorf, Germany).

Article Title: The Pharmacological Potential of Novel Melittin Variants from the Honeybee and Solitary Bees against Inflammation and Cancer
Article Snippet: HEK293T cells were transfected with pGloSensor-22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany).

Article Title: Location-biased activation of the proton-sensor GPR65 is uncoupled from receptor trafficking.
Article Snippet: The following amounts of DNA were used per well: 60 ng of pGloSensor- 22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control, pCDNA3.1+).

Incubation:

Article Title: Compartment-Specific Activation of the Proton-Sensor GPR65 is Uncoupled from Receptor Trafficking
Article Snippet: The following amounts of DNA were used per well: 60 ng of pGloSensor-22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control, pCDNA3.1 + ).

Article Title: SPMs exert anti-inflammatory and pro-resolving effects through positive allosteric modulation of the prostaglandin EP4 receptor
Article Snippet: To generate a lentiviral transfer plasmid (pLV) carrying the cDNA encoding the GloSensor-22F cAMP reporter, the coding sequence of the GloSensor-22F cAMP reporter was subcloned from the pGloSensor-22F cAMP plasmid (Promega, E2301) into the backbone of basic pLV-puro lentiviral expression vector (Vector builder).

Article Title: Diversely evolved xibalbin variants from remipede venom inhibit potassium channels and activate PKA-II and Erk1/2 signaling.
Article Snippet: B) HEK293T cells were transfected with pGloSensor‐22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany). cAMP transfected HEK293T cells were incubated in DMEM without phenol red supplemented with pGlo sensor cAMP reagent (E1290, Promega, Walldorf, Germany).

Article Title: Diversely evolved xibalbin variants from remipede venom inhibit potassium channels and activate PKA-II and Erk1/2 signaling
Article Snippet: B) HEK293T cells were transfected with pGloSensor-22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany). cAMP transfected HEK293T cells were incubated in DMEM without phenol red supplemented with pGlo sensor cAMP reagent (E1290, Promega, Walldorf, Germany).

Article Title: The Pharmacological Potential of Novel Melittin Variants from the Honeybee and Solitary Bees against Inflammation and Cancer
Article Snippet: HEK293T cells were transfected with pGloSensor-22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany).

Article Title: Location-biased activation of the proton-sensor GPR65 is uncoupled from receptor trafficking.
Article Snippet: The following amounts of DNA were used per well: 60 ng of pGloSensor- 22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control, pCDNA3.1+).

Control:

Article Title: Compartment-Specific Activation of the Proton-Sensor GPR65 is Uncoupled from Receptor Trafficking
Article Snippet: The following amounts of DNA were used per well: 60 ng of pGloSensor-22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control, pCDNA3.1 + ).

Article Title: SPMs exert anti-inflammatory and pro-resolving effects through positive allosteric modulation of the prostaglandin EP4 receptor
Article Snippet: To generate a lentiviral transfer plasmid (pLV) carrying the cDNA encoding the GloSensor-22F cAMP reporter, the coding sequence of the GloSensor-22F cAMP reporter was subcloned from the pGloSensor-22F cAMP plasmid (Promega, E2301) into the backbone of basic pLV-puro lentiviral expression vector (Vector builder).

Article Title: Diversely evolved xibalbin variants from remipede venom inhibit potassium channels and activate PKA-II and Erk1/2 signaling.
Article Snippet: B) HEK293T cells were transfected with pGloSensor‐22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany). cAMP transfected HEK293T cells were incubated in DMEM without phenol red supplemented with pGlo sensor cAMP reagent (E1290, Promega, Walldorf, Germany).

Article Title: Diversely evolved xibalbin variants from remipede venom inhibit potassium channels and activate PKA-II and Erk1/2 signaling
Article Snippet: B) HEK293T cells were transfected with pGloSensor-22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany). cAMP transfected HEK293T cells were incubated in DMEM without phenol red supplemented with pGlo sensor cAMP reagent (E1290, Promega, Walldorf, Germany).

Article Title: The Pharmacological Potential of Novel Melittin Variants from the Honeybee and Solitary Bees against Inflammation and Cancer
Article Snippet: HEK293T cells were transfected with pGloSensor-22F cAMP plasmid (E2301, Promega, Walldorf, Germany) using turbofect reagent (Thermofisher Scientific, Frankfurt am Main, Germany).

Article Title: Location-biased activation of the proton-sensor GPR65 is uncoupled from receptor trafficking.
Article Snippet: The following amounts of DNA were used per well: 60 ng of pGloSensor- 22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control, pCDNA3.1+).



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